Individual and population variation in invertebrates revealed by Inter-simple Sequence Repeats (ISSRs)

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Individual and population variation in invertebrates revealed by Inter-simple Sequence Repeats (ISSRs)

PCR-based molecular markers are well suited for questions requiring large scale surveys of plant and animal populations. Inter-simple Sequence Repeats or ISSRs are analyzed by a recently developed technique based on the amplification of the regions between inverse-oriented microsatellite loci with oligonucleotides anchored in microsatellites themselves. ISSRs have shown much promise for the stu...

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Genetic Variation and Population Differentiation in a Medical Herb Houttuynia cordata in China Revealed by Inter-Simple Sequence Repeats (ISSRs)

Houttuynia cordata is an important traditional Chinese herb with unresolved genetics and taxonomy, which lead to potential problems in the conservation and utilization of the resource. Inter-simple sequence repeat (ISSR) markers were used to assess the level and distribution of genetic diversity in 226 individuals from 15 populations of H. cordata in China. ISSR analysis revealed low genetic va...

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Determination of genetic diversity among wild and domesticated beans using Inter Simple Sequence Repeats(ISSRs),

Introduction Many different DNA-based molecular techniques are being developed for fingerprinting and to estimate genetic diversity in eukaryotic organisms. Because of ¿le relative ease in its utilization, PCR is increasingly being used as a tool in marker development. Random amplified polymorphic DNA (RAPD) markers are easier to analyze than RFLPs and are being used in breeding programs to tag...

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Population structure and genetic diversity of Botrychium pumicola (Ophioglossaceae) based on inter-simple sequence repeats (ISSR).

Species of Botrychium reproduce by spores that form subterranean gametophytes and a few, like B. pumicola, also reproduce asexually with subterranean sporophytic gemmae. The goal of this study was to examine the genetic diversity of B. pumicola populations and to better understand the role of gemmae. Ninety-nine individuals from three monitored populations were sampled. The technique of inter-s...

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Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats.

Adoption of inter simple sequence repeats (ISSR) technique to analyze the genetic variability of somatic embryo derived tea plants was evaluated. Morphological characterisation of the field grown plants revealed no identical character aligning with the parent, UPASI-10. Out of 40 primers, 15 exhibited concurrent polymorphism were selected for the study. Genetic variability of somaclones derived...

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ژورنال

عنوان ژورنال: Journal of Insect Science

سال: 2001

ISSN: 0970-3837,1536-2442

DOI: 10.1673/031.001.0108